› 5n23

X-ray diffraction
2.09Å resolution

Protein kinase A mutants as surrogate model for Aurora B with AT9283 inhibitor

Released:
DOI: 10.2210/pdb5n23/pdb
PDB entry 5n23 coloured by chain, front view.
PDB entry 5n23 coloured by chain, side view.
PDB entry 5n23 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Alam KA, Rothweiler U, Engh RA

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148114 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 41.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17612 (Residues: 1-196, 197-351; Coverage: 100%)
Gene names: PKACA, PRKACA
Sequence domains: Protein kinase domain
cAMP-dependent protein kinase inhibitor alpha Chain: B
Molecule details ›
Chain: B
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61925 (Residues: 6-25; Coverage: 26%)
Gene names: PKIA, PRKACN1

Ligands and Environments

1 bound ligand:
Ligand 35R 1 x 35R
2 modified residues:
Ligand SEP 3 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 72Å b: 74.54Å c: 79.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.239
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links