› 5n26

X-ray diffraction
2.05Å resolution

X-ray structure of human heavy chain ferritin in complex with cisplatin

Released:
DOI: 10.2210/pdb5n26/pdb
PDB entry 5n26 coloured by chain, front view.
PDB entry 5n26 coloured by chain, side view.
PDB entry 5n26 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cisplatin Binding Sites in Human H-Chain Ferritin.
Ferraro G, Ciambellotti S, Messori L, Merlino A
Inorg Chem 56 9064-9070 (2017)
PMID: 28737381

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02794 (Residues: 2-183; Coverage: 100%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

4 bound ligands:
Ligand CPT 3 x CPT
Ligand 73M 1 x 73M
Ligand CL 6 x CL
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: F432
Unit cell:
a: 183.069Å b: 183.069Å c: 183.069Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 citation in other articles

New tools for automated high-resolution cryo-EM structure determination in RELION-3.
Zivanov et al. (2018)
12 more

3 mentions without citation

Cryo-EM model validation recommendations based on outcomes of the 2019 EMDataResource challenge.
Lawson et al. (2021)
2 more