› 5n8l

Solution NMR

Structure of TRBP dsRBD 1 and 2 in complex with a 19 bp siRNA (Complex B)

Released:
DOI: 10.2210/pdb5n8l/pdb
PDB entry 5n8l coloured by chain, ensemble of 20 models, front view.
PDB entry 5n8l coloured by chain, ensemble of 20 models, side view.
PDB entry 5n8l coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Structural basis of siRNA recognition by TRBP double-stranded RNA binding domains.
Masliah G, Maris C, König SL, Yulikov M, Aeschimann F, Malinowska AL, Mabille J, Weiler J, Holla A, Hunziker J, Meisner-Kober N, Schuler B, Jeschke G, Allain FH
EMBO J 37 (2018)
PMID: 29449323

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172352 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
RISC-loading complex subunit TARBP2 Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 23.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15633 (Residues: 16-227; Coverage: 58%)
Gene names: TARBP2, TRBP
Sequence domains: Double-stranded RNA binding motif
RNA (5'-R(*UP*UP*AP*AP*UP*UP*AP*UP*CP*UP*AP*UP*UP*CP*CP*GP*UP*AP*CP*UP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.53 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*UP*AP*CP*GP*GP*AP*AP*UP*AP*GP*AP*UP*AP*AP*UP*UP*AP*AP*UP*U)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.75 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 56%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.
Lerner et al. (2021)
5 more

6 mentions without citation

A Structural View of miRNA Biogenesis and Function.
Leitão et al. (2022)
5 more