› 5naz

X-ray diffraction
1.85Å resolution

Crystal structures of homooligomers of collagen type IV. alpha5NC1

Released:
DOI: 10.2210/pdb5naz/pdb
PDB entry 5naz coloured by chain, front view.
PDB entry 5naz coloured by chain, side view.
PDB entry 5naz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of collagen IV globular domains: insight into associated pathologies, folding and network assembly.
Casino P, Gozalbo-Rovira R, Rodríguez-Díaz J, Banerjee S, Boutaud A, Rubio V, Hudson BG, Saus J, Cervera J, Marina A
IUCrJ 5 765-779 (2018)
PMID: 30443360

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Collagen alpha-5(IV) chain Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29400 (Residues: 1457-1685; Coverage: 14%)
Gene name: COL4A5
Sequence domains: C-terminal tandem repeated domain in type 4 procollagen

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P4132
Unit cell:
a: 121.283Å b: 121.283Å c: 121.283Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.19
Expression system: Spodoptera frugiperda

Quick links

Citations

7 citation in other articles

A chloride ring is an ancient evolutionary innovation mediating the assembly of the collagen IV scaffold of basement membranes.
Pedchenko et al. (2019)
6 more

5 mentions without citation

Development of an exon skipping therapy for X-linked Alport syndrome with truncating variants in COL4A5.
Yamamura et al. (2020)
4 more