› 5nb2

X-ray diffraction
2.5Å resolution

Crystal structures of homooligomers of collagen type IV. alpha2NC1

Released:
DOI: 10.2210/pdb5nb2/pdb
PDB entry 5nb2 coloured by chain, front view.
PDB entry 5nb2 coloured by chain, side view.
PDB entry 5nb2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of collagen IV globular domains: insight into associated pathologies, folding and network assembly.
Casino P, Gozalbo-Rovira R, Rodríguez-Díaz J, Banerjee S, Boutaud A, Rubio V, Hudson BG, Saus J, Cervera J, Marina A
IUCrJ 5 765-779 (2018)
PMID: 30443360

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Canstatin Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.11 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08572 (Residues: 1485-1712; Coverage: 14%)
Gene name: COL4A2
Sequence domains: C-terminal tandem repeated domain in type 4 procollagen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: I422
Unit cell:
a: 94.33Å b: 94.33Å c: 225.618Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.246 0.279
Expression system: Spodoptera frugiperda

Quick links

Citations

7 citation in other articles

A chloride ring is an ancient evolutionary innovation mediating the assembly of the collagen IV scaffold of basement membranes.
Pedchenko et al. (2019)
6 more

1 mention without citation

Structures of collagen IV globular domains: insight into associated pathologies, folding and network assembly.
Casino et al. (2018)