› 5nbq

X-ray diffraction
3.18Å resolution

The structure of the tripartite complex between OspE, the C-terminal domains of factor H and C3dg

Released:
DOI: 10.2210/pdb5nbq/pdb
PDB entry 5nbq coloured by chain, front view.
PDB entry 5nbq coloured by chain, side view.
PDB entry 5nbq coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a tripartite complex between C3dg, C-terminal domains of factor H and OspE of Borrelia burgdorferi.
Kolodziejczyk R, Mikula KM, Kotila T, Postis VLG, Jokiranta TS, Goldman A, Meri T
PLoS One 12 e0188127 (2017)
PMID: 29190743

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133842 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Complement C3d fragment Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 294 amino acids
Theoretical weight: 32.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01024 (Residues: 994-1287; Coverage: 18%)
Gene names: C3, CPAMD1
Sequence domains: A-macroglobulin TED domain
Structure domains: Glycosyltransferase
Complement factor H Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 127 amino acids
Theoretical weight: 14.44 KDa
Source organism: Homo sapiens
Expression system: Pichia
UniProt:
  • Canonical: P08603 (Residues: 1104-1230; Coverage: 11%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)
Structure domains: Complement Module, domain 1
Outer surface protein E Chains: G, I
Molecule details ›
Chains: G, I
Length: 129 amino acids
Theoretical weight: 14.39 KDa
Source organism: Borreliella burgdorferi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45001 (Residues: 42-170; Coverage: 75%)
Gene name: OspE
Sequence domains: Borrelia outer surface protein E
Structure domains: Borrelia outer surface protein E/F
Outer surface protein E Chain: H
Molecule details ›
Chain: H
Length: 134 amino acids
Theoretical weight: 14.81 KDa
Source organism: Borreliella burgdorferi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45001 (Residues: 42-170; Coverage: 75%)
Gene name: OspE
Sequence domains: Borrelia outer surface protein E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21212
Unit cell:
a: 124.907Å b: 165.063Å c: 84.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.233 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Pichia
  • Escherichia coli

Quick links

Citations

4 review citations

Hijacking Factor H for Complement Immune Evasion.
Moore et al. (2021)
3 more

7 mentions without citation

Complement Evasion by Lyme Disease Spirochetes.
Skare et al. (2020)
6 more