› 5nfd

X-ray diffraction
2.18Å resolution

Antiparallel monomeric coiled coil of Kif21A

Released:
DOI: 10.2210/pdb5nfd/pdb
PDB entry 5nfd coloured by chain, front view.
PDB entry 5nfd coloured by chain, side view.
PDB entry 5nfd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Combinatorial use of disulfide bridges and native sulfur-SAD phasing for rapid structure determination of coiled-coils.
Kraatz SHW, Bianchi S, Steinmetz MO
Biosci Rep 38 (2018)
PMID: 30135143

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF21A Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7Z4S6 (Residues: 938-1017; Coverage: 5%)
Gene names: KIAA1708, KIF2, KIF21A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 33.97Å b: 41.67Å c: 123.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.197 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Addressing the Molecular Mechanism of Longitudinal Lamin Assembly Using Chimeric Fusions.
Stalmans et al. (2020)
3 more

2 mentions without citation

LIVE-PAINT allows super-resolution microscopy inside living cells using reversible peptide-protein interactions.
Oi et al. (2020)
1 more