› 5nfr

X-ray diffraction
2.4Å resolution

Crystal structure of malate dehydrogenase from Plasmodium falciparum (PfMDH)

Released:
DOI: 10.2210/pdb5nfr/pdb
PDB entry 5nfr coloured by chain, front view.
PDB entry 5nfr coloured by chain, side view.
PDB entry 5nfr coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Oligomeric interfaces as a tool in drug discovery: Specific interference with activity of malate dehydrogenase of Plasmodium falciparum in vitro.
Lunev S, Butzloff S, Romero AR, Linzke M, Batista FA, Meissner KA, Müller IB, Adawy A, Wrenger C, Groves MR
PLoS One 13 e0195011 (2018)
PMID: 29694407

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111618 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 320 amino acids
Theoretical weight: 34.96 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: C6KT25 (Residues: 1-313; Coverage: 100%)
Gene name: PF3D7_0618500
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CIT 15 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P1
Unit cell:
a: 72.018Å b: 152.689Å c: 158.393Å
α: 103.77° β: 101.46° γ: 94.93°
R-values:
R R work R free
0.249 0.248 0.264
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Metabolic changes accompanying the loss of fumarate hydratase and malate-quinone oxidoreductase in the asexual blood stage of Plasmodium falciparum.
Rajaram et al. (2022)
1 more

5 mentions without citation

Roles of Virtual Screening and Molecular Dynamics Simulations in Discovering and Understanding Antimalarial Drugs.
Duay et al. (2023)
4 more