› 5nfu

X-ray diffraction
1.81Å resolution

The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with LHSpTA peptide

Released:
DOI: 10.2210/pdb5nfu/pdb
PDB entry 5nfu coloured by chain, front view.
PDB entry 5nfu coloured by chain, side view.
PDB entry 5nfu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A cryptic hydrophobic pocket in the polo-box domain of the polo-like kinase PLK1 regulates substrate recognition and mitotic chromosome segregation.
Sharma P, Mahen R, Rossmann M, Stokes JE, Hardwick B, Huggins DJ, Emery A, Kunciw DL, Hyvönen M, Spring DR, McKenzie GJ, Venkitaraman AR
Sci Rep 9 15930 (2019)
PMID: 31685831

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156783 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 27.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 371-602; Coverage: 39%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain
Ac-LEU-HIS-SER-(TPO)-ALA Chain: C
Molecule details ›
Chain: C
Length: 5 amino acids
Theoretical weight: 651 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand LAY 1 x LAY
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 35.197Å b: 59.808Å c: 58.256Å
α: 90° β: 102.69° γ: 90°
R-values:
R R work R free
0.17 0.168 0.202
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Orchestrating serine/threonine phosphorylation and elucidating downstream effects by short linear motifs.
Kliche et al. (2022)
1 more