› 5ngv

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE Activin receptor type-2B LIGAND BINDING DOMAIN IN COMPLEX WITH BIMAGRUMAB FV, ORTHORHOMBIC CRYSTAL FORM

Released:
DOI: 10.2210/pdb5ngv/pdb
PDB entry 5ngv coloured by chain, front view.
PDB entry 5ngv coloured by chain, side view.
PDB entry 5ngv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy.
Morvan F, Rondeau JM, Zou C, Minetti G, Scheufler C, Scharenberg M, Jacobi C, Brebbia P, Ritter V, Toussaint G, Koelbing C, Leber X, Schilb A, Witte F, Lehmann S, Koch E, Geisse S, Glass DJ, Lach-Trifilieff E
Proc Natl Acad Sci U S A 114 12448-12453 (2017)
PMID: 29109273

Function and Biology Details

Reaction catalysed: 
ATP + [receptor-protein] = ADP + [receptor-protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212013 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Activin receptor type-2B Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 11.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13705 (Residues: 24-117; Coverage: 19%)
Gene name: ACVR2B
Sequence domains: Activin types I and II receptor domain
Structure domains: CD59
anti-human ActRIIB mAb BYM338 heavy-chain Chain: H
Molecule details ›
Chain: H
Length: 121 amino acids
Theoretical weight: 13.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
anti-human ActRIIB mAb BYM338 light-chain Chain: L
Molecule details ›
Chain: L
Length: 119 amino acids
Theoretical weight: 12.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand PG4 1 x PG4
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I222
Unit cell:
a: 62.1Å b: 114.17Å c: 117.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.198
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Role of Myokines in Regulating Skeletal Muscle Mass and Function.
Lee et al. (2019)
21 more

1 mention without citation

Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy.
Morvan et al. (2017)