› 5nht

X-ray diffraction
3.2Å resolution

human 199.54-16 TCR in complex with Melan-A/MART-1 (26-35) peptide and HLA-A2

Released:
DOI: 10.2210/pdb5nht/pdb
PDB entry 5nht coloured by chain, front view.
PDB entry 5nht coloured by chain, side view.
PDB entry 5nht coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Exertier C, Reiser J-B, Lantez V, Chouquet A, Bonneville M, Saulquin X, Housset D

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-100459 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: H
Molecule details ›
Chain: H
Length: 276 amino acids
Theoretical weight: 31.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: L
Molecule details ›
Chain: L
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Melanoma antigen recognized by T-cells 1 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 985 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16655 (Residues: 26-35; Coverage: 9%)
Gene names: MART1, MLANA
Ig-like domain-containing protein; T cell receptor alpha variable 12-2 Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 23.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K7N5N2 (Residues: 1-1, 96-205; Coverage: 54%)
  • Canonical: A0A075B6T6 (Residues: 21-112; Coverage: 99%)
Gene name: TRAV12-2
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 251 amino acids
Theoretical weight: 28.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5B3 (Residues: 22-113; Coverage: 99%)
  • Canonical: A0A0C4ZKA8 (Residues: 31-174; Coverage: 83%)
Gene names: TCRBV17S1A1T, TRB, TRBV19
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand SCN 1 x SCN
Ligand K 2 x K
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 222.077Å b: 49.284Å c: 96.133Å
α: 90° β: 97.61° γ: 90°
R-values:
R R work R free
0.217 0.213 0.299
Expression systems:
  • Escherichia coli
  • Not provided
  • Escherichia coli BL21(DE3)

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