› 5nin

X-ray diffraction
1.7Å resolution

Crystal Structure of AKAP79 calmodulin binding domain peptide in complex with Ca2+/Calmodulin

Released:
DOI: 10.2210/pdb5nin/pdb
PDB entry 5nin coloured by chain, front view.
PDB entry 5nin coloured by chain, side view.
PDB entry 5nin coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of AKAP79 regulation by calmodulin.
Patel N, Stengel F, Aebersold R, Gold MG
Nat Commun 8 1681 (2017)
PMID: 29162807

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143916 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
A-kinase anchor protein 5 Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 amino acids
Theoretical weight: 1.89 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P24588 (Residues: 77-92; Coverage: 4%)
Gene names: AKAP5, AKAP79
Sequence domains: WSK motif

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P42212
Unit cell:
a: 76.46Å b: 76.46Å c: 128.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.194
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Postsynaptic localization and regulation of AMPA receptors and Cav1.2 by β2 adrenergic receptor/PKA and Ca2+/CaMKII signaling.
Patriarchi et al. (2018)
1 more

2 mentions without citation

Molecular basis of AKAP79 regulation by calmodulin.
Patel et al. (2017)
1 more