› 5nkp

X-ray diffraction
2.8Å resolution

Crystal structure of the human KLHL3 Kelch domain in complex with a WNK3 peptide

Released:
DOI: 10.2210/pdb5nkp/pdb
PDB entry 5nkp coloured by chain, front view.
PDB entry 5nkp coloured by chain, side view.
PDB entry 5nkp coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Chen Z, Sorrell FJ, Pinkas DM, Williams E, Mathea S, Goubin S, von Delft F, Arrowsmith CH, Edwards AM, Burgess-Brown N, Bountra C, Bullock A

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189841 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kelch-like protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 31.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UH77 (Residues: 298-587; Coverage: 49%)
Gene names: KIAA1129, KLHL3
Sequence domains: Kelch motif
Structure domains: Kelch-type beta propeller
Serine/threonine-protein kinase WNK3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9BYP7 (Residues: 537-547; Coverage: 1%)
Gene names: KIAA1566, PRKWNK3, WNK3

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 84.66Å b: 169.72Å c: 123.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.247
Expression systems:
  • Escherichia coli
  • Not provided

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