› 5nmi

X-ray diffraction
3.5Å resolution

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-132264 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: A, N
Molecule details ›
Chains: A, N
Length: 444 amino acids
Theoretical weight: 49.02 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P31800 (Residues: 36-479; Coverage: 93%)
Gene name: UQCRC1
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: B, O
Molecule details ›
Chains: B, O
Length: 423 amino acids
Theoretical weight: 45.02 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P23004 (Residues: 31-453; Coverage: 93%)
Gene name: UQCRC2
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Cytochrome b Chains: C, P
Molecule details ›
Chains: C, P
Length: 372 amino acids
Theoretical weight: 41.79 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00157 (Residues: 8-379; Coverage: 98%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1, heme protein, mitochondrial Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 240 amino acids
Theoretical weight: 27.24 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00125 (Residues: 86-325; Coverage: 74%)
Gene name: CYC1
Sequence domains: Cytochrome C1 family
Structure domains:
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: E, I, R, V
Molecule details ›
Chains: E, I, R, V
Length: 274 amino acids
Theoretical weight: 29.59 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13272 (Residues: 1-274; Coverage: 100%)
Gene name: UQCRFS1
Sequence domains:
Structure domains:
Cytochrome b-c1 complex subunit 7 Chains: F, S
Molecule details ›
Chains: F, S
Length: 111 amino acids
Theoretical weight: 13.5 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00129 (Residues: 1-111; Coverage: 100%)
Gene name: UQCRB
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Structure domains: Cytochrome b-c1 complex subunit 7
Cytochrome b-c1 complex subunit 8 Chains: G, T
Molecule details ›
Chains: G, T
Length: 82 amino acids
Theoretical weight: 9.74 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13271 (Residues: 1-82; Coverage: 100%)
Gene name: UQCRQ
Sequence domains: UcrQ family
Structure domains: Cytochrome b-c1 complex subunit 8
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: H, U
Molecule details ›
Chains: H, U
Length: 91 amino acids
Theoretical weight: 10.64 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00126 (Residues: 1-91; Coverage: 100%)
Gene name: UQCRH
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Structure domains: Ubiquinol-cytochrome C reductase hinge domain
Cytochrome b-c1 complex subunit 9 Chains: J, W
Molecule details ›
Chains: J, W
Length: 64 amino acids
Theoretical weight: 7.47 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00130 (Residues: 1-64; Coverage: 100%)
Gene name: UQCR10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Structure domains: Cytochrome b-c1 complex subunit 9
Cytochrome b-c1 complex subunit 10 Chains: K, X
Molecule details ›
Chains: K, X
Length: 22 amino acids
Theoretical weight: 2.25 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07552 (Residues: 15-36; Coverage: 39%)
Gene names: UQCR, UQCR11
Sequence domains: Ubiquinol-cytochrome C reductase complex, 6.4kD protein

Ligands and Environments

6 bound ligands:
Ligand HEM 4 x HEM
Ligand MJM 2 x MJM
Ligand PEE 6 x PEE
Ligand CDL 6 x CDL
Ligand HEC 2 x HEC
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P65
Unit cell:
a: 129.477Å b: 129.477Å c: 720.294Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.248 0.268

Quick links

Citations

7 review citations

Drugs in development for toxoplasmosis: advances, challenges, and current status.
Alday et al. (2017)
6 more

4 mentions without citation

MitImpact 3: modeling the residue interaction network of the Respiratory Chain subunits.
Castellana et al. (2021)
3 more