› 5nmv

X-ray diffraction
1.65Å resolution

Crystal structure of 2F22 Fab fragment against TFPI1

Released:
DOI: 10.2210/pdb5nmv/pdb
PDB entry 5nmv coloured by chain, front view.
PDB entry 5nmv coloured by chain, side view.
PDB entry 5nmv coloured by chain, top view.
Source organisms:
Primary publication:
Factor Xa and VIIa inhibition by Tissue Factor Pathway Inhibitor is prevented by a monoclonal antibody to its Kunitz-1 domain.
Augustsson C, Svensson A, Kjaer B, Chao TY, Wenjuan X, Krogh BO, Breinholt J, Clausen JT, Hilden I, Petersen HH, Petersen LC
J. Thromb. Haemost. (2018)
PMID: 29532595

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210488 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.24 KDa
Source organism: Mus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.81 KDa
Source organism: Mus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Tissue factor pathway inhibitor Chain: K
Molecule details ›
Chain: K
Length: 86 amino acids
Theoretical weight: 9.8 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P10646 (Residues: 29-107; Coverage: 29%)
Gene names: LACI, TFPI, TFPI1
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: C2
Unit cell:
a: 89.01Å b: 66.66Å c: 106.11Å
α: 90° β: 111.18° γ: 90°
R-values:
R R work R free
0.177 0.175 0.202
Expression system: Homo sapiens

Quick links

Citations

2 citation in other articles

NMR structure determination of Ixolaris and factor X(a) interaction reveals a noncanonical mechanism of Kunitz inhibition.
De Paula et al. (2019)
1 more