› 5nq3

X-ray diffraction
1.57Å resolution

'Porcine (Sus scrofa) Major Histocompatibility Complex, class I, with human beta2 micro globulin, presenting EFEDLTFLA

Released:
DOI: 10.2210/pdb5nq3/pdb
PDB entry 5nq3 coloured by chain, front view.
PDB entry 5nq3 coloured by chain, side view.
PDB entry 5nq3 coloured by chain, top view.
Source organisms:
Primary publication:
Induction of influenza-specific local CD8 T-cells in the respiratory tract after aerosol delivery of vaccine antigen or virus in the Babraham inbred pig.
Tungatt K, Dolton G, Morgan SB, Attaf M, Fuller A, Whalley T, Hemmink JD, Porter E, Szomolay B, Montoya M, Hammond JA, Miles JJ, Cole DK, Townsend A, Bailey M, Rizkallah PJ, Charleston B, Tchilian E, Sewell AK
PLoS Pathog 14 e1007017 (2018)
PMID: 29772011

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-109391 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 276 amino acids
Theoretical weight: 31.68 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: B1PJV3 (Residues: 22-296; Coverage: 81%)
Gene name: SLA-1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Nucleoprotein Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.08 KDa
Source organism: unidentified influenza virus
Expression system: Not provided
UniProt:
  • Canonical: P03466 (Residues: 252-260; Coverage: 2%)
Gene name: NP

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 59.301Å b: 59.696Å c: 66.443Å
α: 81.48° β: 67.62° γ: 68.6°
R-values:
R R work R free
0.22 0.218 0.262
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Rethinking next-generation vaccines for coronaviruses, influenzaviruses, and other respiratory viruses.
Morens et al. (2023)
5 more

1 mention without citation

Induction of influenza-specific local CD8 T-cells in the respiratory tract after aerosol delivery of vaccine antigen or virus in the Babraham inbred pig.
Tungatt et al. (2018)