› 5nqp

X-ray diffraction
2.86Å resolution

Structure of a fHbp(V1.4):PorA(P1.16) chimera. Fusion at fHbp position 151.

Released:
DOI: 10.2210/pdb5nqp/pdb
PDB entry 5nqp coloured by chain, front view.
PDB entry 5nqp coloured by chain, side view.
PDB entry 5nqp coloured by chain, top view.
Source organisms:
Primary publication:
Structure-based design of chimeric antigens for multivalent protein vaccines.
Hollingshead S, Jongerius I, Exley RM, Johnson S, Lea SM, Tang CM
Nat Commun 9 1051 (2018)
PMID: 29535307

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Factor H binding protein variant B16_004; Major outer membrane protein P.IA Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 271 amino acids
Theoretical weight: 29.43 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q6VS06 (Residues: 7-85, 87-255; Coverage: 97%)
  • Canonical: P13415 (Residues: 195-208; Coverage: 4%)
Gene names: fhbp, porA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 11 x EDO
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2221
Unit cell:
a: 100.72Å b: 171.42Å c: 131.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.239
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure-Based Vaccine Antigen Design.
Graham et al. (2019)
6 more

2 mentions without citation

Structure-based design of chimeric antigens for multivalent protein vaccines.
Hollingshead et al. (2018)
1 more