› 5nqx

X-ray diffraction
3.66Å resolution

Structure of a fHbp(V1.1):PorA(P1.16) chimera. Fusion at fHbp position 294.

Released:
DOI: 10.2210/pdb5nqx/pdb
PDB entry 5nqx coloured by chain, front view.
PDB entry 5nqx coloured by chain, side view.
PDB entry 5nqx coloured by chain, top view.
Source organisms:
Primary publication:
Structure-based design of chimeric antigens for multivalent protein vaccines.
Hollingshead S, Jongerius I, Exley RM, Johnson S, Lea SM, Tang CM
Nat Commun 9 1051 (2018)
PMID: 29535307

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Factor H binding protein; Major outer membrane protein P.IA Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 269 amino acids
Theoretical weight: 29.18 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JXV4 (Residues: 26-247, 249-274; Coverage: 97%)
  • Canonical: P13415 (Residues: 195-207; Coverage: 4%)
Gene names: NMB1870, fhbP, porA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Unit cell:
a: 94.85Å b: 114.9Å c: 160.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.282
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Structure-Based Vaccine Antigen Design.
Graham et al. (2019)
6 more

2 mentions without citation

Structure-based design of chimeric antigens for multivalent protein vaccines.
Hollingshead et al. (2018)
1 more