› 5nt1

X-ray diffraction
2.82Å resolution

Complex of influenza A NS1 effector domain with TRIM25 coiled coil

Released:
DOI: 10.2210/pdb5nt1/pdb
PDB entry 5nt1 coloured by chain, front view.
PDB entry 5nt1 coloured by chain, side view.
PDB entry 5nt1 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition.
Koliopoulos MG, Lethier M, van der Veen AG, Haubrich K, Hennig J, Kowalinski E, Stevens RV, Martin SR, Reis e Sousa C, Cusack S, Rittinger K
Nat Commun 9 1820 (2018)
PMID: 29739942

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + [ISG15] + [protein]-lysine = AMP + diphosphate + [protein]-N-ISGyllysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137078 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin/ISG15 ligase TRIM25 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 193 amino acids
Theoretical weight: 22.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14258 (Residues: 190-379; Coverage: 30%)
Gene names: EFP, RNF147, TRIM25, ZNF147
Non-structural protein 1 Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 154 amino acids
Theoretical weight: 17.08 KDa
Source organism: Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: P03496 (Residues: 80-230; Coverage: 66%)
Gene name: NS
Sequence domains: Influenza non-structural protein (NS1)
Structure domains: Influenza virus non-structural protein, effector domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C222
Unit cell:
a: 131.38Å b: 225.23Å c: 146.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.224
Expression system: Escherichia coli

Quick links

Citations

33 review citations

RIG-I-like receptors: their regulation and roles in RNA sensing.
Rehwinkel et al. (2020)
32 more

1 mention without citation

Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.
Stoll et al. (2019)