› 5nvg

X-ray diffraction
1.07Å resolution

Thr12 Phosphorylated Ubiquitin

Released:
DOI: 10.2210/pdb5nvg/pdb
PDB entry 5nvg coloured by chain, front view.
PDB entry 5nvg coloured by chain, side view.
PDB entry 5nvg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing.
Zhang MS, Brunner SF, Huguenin-Dezot N, Liang AD, Schmied WH, Rogerson DT, Chin JW
Nat Methods 14 729-736 (2017)
PMID: 28553966

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand PEG 1 x PEG
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 50.46Å b: 24.74Å c: 47.65Å
α: 90° β: 113.17° γ: 90°
R-values:
R R work R free
0.131 0.129 0.167
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Expanding and reprogramming the genetic code.
Chin JW. (2017)
28 more