› 5nvk

X-ray diffraction
2.9Å resolution

Crystal structure of the human 4EHP-GIGYF1 complex

Released:
DOI: 10.2210/pdb5nvk/pdb
PDB entry 5nvk coloured by chain, front view.
PDB entry 5nvk coloured by chain, side view.
PDB entry 5nvk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression.
Peter D, Weber R, Sandmeir F, Wohlbold L, Helms S, Bawankar P, Valkov E, Igreja C, Izaurralde E
Genes Dev 31 1147-1161 (2017)
PMID: 28698298

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130035 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E type 2 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 189 amino acids
Theoretical weight: 21.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60573 (Residues: 52-234; Coverage: 75%)
Gene names: EIF4E2, EIF4EL3
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
GRB10-interacting GYF protein 1 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 75 amino acids
Theoretical weight: 8.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75420 (Residues: 33-103; Coverage: 7%)
Gene names: CDS2, GIGYF1, PERQ1, PP3360

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P42
Unit cell:
a: 135.32Å b: 135.32Å c: 60.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Getting to the Cores of Autism.
Iakoucheva et al. (2019)
5 more

1 mention without citation

GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression.
Peter et al. (2017)