› 5nx2

X-ray diffraction
3.7Å resolution

Crystal structure of thermostabilised full-length GLP-1R in complex with a truncated peptide agonist at 3.7 A resolution

Released:
DOI: 10.2210/pdb5nx2/pdb
PDB entry 5nx2 coloured by chain, front view.
PDB entry 5nx2 coloured by chain, side view.
PDB entry 5nx2 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the GLP-1 receptor bound to a peptide agonist.
Jazayeri A, Rappas M, Brown AJH, Kean J, Errey JC, Robertson NJ, Fiez-Vandal C, Andrews SP, Congreve M, Bortolato A, Mason JS, Baig AH, Teobald I, Doré AS, Weir M, Cooke RM, Marshall FH
Nature 546 254-258 (2017)
PMID: 28562585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154991 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Glucagon-like peptide 1 receptor Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 49.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43220 (Residues: 24-432; Coverage: 93%)
Gene name: GLP1R
Sequence domains:
truncated peptide agonist Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.49 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand SOG 3 x SOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3121
Unit cell:
a: 94.435Å b: 94.435Å c: 163.905Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.287 0.285 0.334
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

22 review citations

Glucagon-like peptide 1 (GLP-1).
Müller et al. (2019)
21 more

28 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
27 more