› 5o10

X-ray diffraction
1.36Å resolution

Y48H mutant of human cytochrome c

Released:
DOI: 10.2210/pdb5o10/pdb
PDB entry 5o10 coloured by chain, front view.
PDB entry 5o10 coloured by chain, side view.
PDB entry 5o10 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Heightened Dynamics of the Oxidized Y48H Variant of Human Cytochrome c Increases Its Peroxidatic Activity.
Deacon OM, Karsisiotis AI, Moreno-Chicano T, Hough MA, Macdonald C, Blumenschein TMA, Wilson MT, Moore GR, Worrall JAR
Biochemistry 56 6111-6124 (2017)
PMID: 29083920

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 11.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P99999 (Residues: 2-105; Coverage: 99%)
Gene names: CYC, CYCS
Sequence domains: Cytochrome c

Ligands and Environments

1 bound ligand:
Ligand HEC 2 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 56.937Å b: 36.498Å c: 60.7Å
α: 90° β: 116.65° γ: 90°
R-values:
R R work R free
0.146 0.144 0.175
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

4 review citations

Tissue-specific regulation of cytochrome c by post-translational modifications: respiration, the mitochondrial membrane potential, ROS, and apoptosis.
Kalpage et al. (2019)
3 more