› 5o32

X-ray diffraction
4.21Å resolution

The structure of complement complex

Released:
DOI: 10.2210/pdb5o32/pdb
PDB entry 5o32 coloured by chain, front view.
PDB entry 5o32 coloured by chain, side view.
PDB entry 5o32 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulator-dependent mechanisms of C3b processing by factor I allow differentiation of immune responses.
Xue X, Wu J, Ricklin D, Forneris F, Di Crescenzio P, Schmidt CQ, Granneman J, Sharp TH, Lambris JD, Gros P
Nat Struct Mol Biol 24 643-651 (2017)
PMID: 28671664

Function and Biology Details

Reaction catalysed: 
Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133854 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (7 distinct):
Complement C3 beta chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 23-667; Coverage: 39%)
Gene names: C3, CPAMD1
Sequence domains:
Complement C3b alpha' chain Chains: B, F
Molecule details ›
Chains: B, F
Length: 915 amino acids
Theoretical weight: 104.07 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 749-1663; Coverage: 56%)
Gene names: C3, CPAMD1
Sequence domains:
Complement factor H Chains: C, G
Molecule details ›
Chains: C, G
Length: 383 amino acids
Theoretical weight: 42.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08603 (Residues: 19-264, 1107-387; Coverage: 31%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)
Complement factor I heavy chain Chains: D, H
Molecule details ›
Chains: D, H
Length: 321 amino acids
Theoretical weight: 35.96 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P05156 (Residues: 19-339; Coverage: 57%)
Gene names: CFI, IF
Sequence domains:
Complement factor I light chain Chains: I, J
Molecule details ›
Chains: I, J
Length: 244 amino acids
Theoretical weight: 27.62 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P05156 (Residues: 340-583; Coverage: 43%)
Gene names: CFI, IF
Sequence domains: Trypsin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand CA 6 x CA
Ligand NAG 12 x NAG
Ligand MLI 1 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 116.37Å b: 122.79Å c: 157.2Å
α: 69.764° β: 76.101° γ: 70.13°
R-values:
R R work R free
0.23 0.229 0.254
Expression system: Homo sapiens

Quick links

Citations

11 review citations

The properdin pathway: an "alternative activation pathway" or a "critical amplification loop" for C3 and C5 activation?
Harrison RA. (2018)
10 more

26 mentions without citation

Novel mechanisms and functions of complement.
Hajishengallis et al. (2017)
25 more