› 5o8k

X-ray diffraction
1.8Å resolution

Crystal structure of mammalian Rev7 in complex with Rev3 1875-1895

Released:
DOI: 10.2210/pdb5o8k/pdb
PDB entry 5o8k coloured by chain, front view.
PDB entry 5o8k coloured by chain, side view.
PDB entry 5o8k coloured by chain, top view.
Source organisms:
Entry authors: Emamzadah S, Tropia L, Huber F, Halazonetis T

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130059 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2B Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 24.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9D752 (Residues: 1-211; Coverage: 100%)
Gene names: Mad2b, Mad2l2, Rev7
Sequence domains: HORMA domain
DNA polymerase zeta catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 28 amino acids
Theoretical weight: 3.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O60673 (Residues: 1873-1898; Coverage: 1%)
Gene names: POLZ, REV3, REV3L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P32
Unit cell:
a: 69.014Å b: 69.014Å c: 42.868Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.255 0.289
Expression system: Escherichia coli BL21

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