› 5oab

X-ray diffraction
1.11Å resolution

A novel crystal form of human RNase6 at atomic resolution

Released:
DOI: 10.2210/pdb5oab/pdb
PDB entry 5oab coloured by chain, front view.
PDB entry 5oab coloured by chain, side view.
PDB entry 5oab coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterization of an RNase with two catalytic centers. Human RNase6 catalytic and phosphate-binding site arrangement favors the endonuclease cleavage of polymeric substrates.
Prats-Ejarque G, Blanco JA, Salazar VA, Nogués VM, Moussaoui M, Boix E
Biochim Biophys Acta Gen Subj 1863 105-117 (2019)
PMID: 30287244

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease K6 Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93091 (Residues: 24-150; Coverage: 100%)
Gene names: RNASE6, RNS6
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

4 bound ligands:
Ligand PO4 6 x PO4
Ligand NA 3 x NA
Ligand CL 3 x CL
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: I23
Unit cell:
a: 95.405Å b: 95.405Å c: 95.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.118 0.117 0.141
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2.
Li et al. (2020)
3 more

1 mention without citation

A Common Polymorphism in RNASE6 Impacts Its Antimicrobial Activity toward Uropathogenic Escherichia coli.
Anguita et al. (2024)