› 5oc7

X-ray diffraction
1.65Å resolution

Crystal structure of the pleckstrin-homology domain of Bcr-Abl in complex with monobody Mb(Bcr-PH_4).

Released:
DOI: 10.2210/pdb5oc7/pdb
PDB entry 5oc7 coloured by chain, front view.
PDB entry 5oc7 coloured by chain, side view.
PDB entry 5oc7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.
Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O
Nat Commun 8 2101 (2017)
PMID: 29235475

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
monomeric
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145739 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Breakpoint cluster region protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 133 amino acids
Theoretical weight: 15.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11274 (Residues: 704-893; Coverage: 10%)
Gene names: BCR, BCR1, D22S11
Sequence domains: PH domain
monobody Mb(Bcr-PH_4) Chains: B, C
Molecule details ›
Chains: B, C
Length: 92 amino acids
Theoretical weight: 9.63 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 7 x GOL
Ligand IP2 1 x IP2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 29.683Å b: 62.606Å c: 67.378Å
α: 62.74° β: 84.77° γ: 89.29°
R-values:
R R work R free
0.175 0.174 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Chronic Myeloid Leukemia: Modern therapies, current challenges and future directions.
Osman et al. (2021)
2 more

3 mentions without citation

Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.
Reckel et al. (2017)
2 more