› 5oeo

Solution NMR

Solution structure of the complex of TRPV5(655-725) with a Calmodulin E32Q/E68Q double mutant

Released:
DOI: 10.2210/pdb5oeo/pdb
PDB entry 5oeo coloured by chain, ensemble of 20 models, front view.
PDB entry 5oeo coloured by chain, ensemble of 20 models, side view.
PDB entry 5oeo coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
The Structural Basis of Calcium-Dependent Inactivation of the Transient Receptor Potential Vanilloid 5 Channel.
Bokhovchuk FM, Bate N, Kovalevskaya NV, Goult BT, Spronk CAEM, Vuister GW
Biochemistry 57 2623-2635 (2018)
PMID: 29584409

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143962 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Transient receptor potential cation channel subfamily V member 5 Chain: C
Molecule details ›
Chain: C
Length: 73 amino acids
Theoretical weight: 7.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NQA5 (Residues: 655-725; Coverage: 10%)
Gene names: ECAC1, TRPV5

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 75%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Structural insights into the gating mechanisms of TRPV channels.
Pumroy et al. (2020)
2 more