› 5ogc

Electron Microscopy
4.8Å resolution

Molecular basis of human kinesin-8 function and inhibition

Released:
DOI: 10.2210/pdb5ogc/pdb
PDB entry 5ogc coloured by chain, ensemble of 2 models, front view.
PDB entry 5ogc coloured by chain, ensemble of 2 models, side view.
PDB entry 5ogc coloured by chain, ensemble of 2 models, top view.
Source organisms:
Primary publication:
Structural basis of human kinesin-8 function and inhibition.
Locke J, Joseph AP, Peña A, Möckel MM, Mayer TU, Topf M, Moores CA
Proc Natl Acad Sci U S A 114 E9539-E9548 (2017)
PMID: 29078367
Related structures: EMD-3803

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135912 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kinesin-like protein KIF18A Chain: K
Molecule details ›
Chain: K
Length: 377 amino acids
Theoretical weight: 42.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NI77 (Residues: 1-374; Coverage: 42%)
Gene names: KIF18A, OK/SW-cl.108
Sequence domains: Kinesin motor domain
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand 9V5 1 x 9V5
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-3803
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

These motors were made for walking.
Hunter et al. (2020)
4 more

1 mention without citation

Structural basis of human kinesin-8 function and inhibition.
Locke et al. (2017)