› 5ohq

X-ray diffraction
1.1Å resolution

Crystal structure of the KOW6-KOW7 domain of human DSIF

Released:
DOI: 10.2210/pdb5ohq/pdb
PDB entry 5ohq coloured by chain, front view.
PDB entry 5ohq coloured by chain, side view.
PDB entry 5ohq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp.
Bernecky C, Plitzko JM, Cramer P
Nat Struct Mol Biol 24 809-815 (2017)
PMID: 28892040

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation factor SPT5 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 12.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00267 (Residues: 979-1087; Coverage: 10%)
Gene names: SPT5, SPT5H, SUPT5H

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 35.091Å b: 75.657Å c: 96.749Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.122 0.14
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Organization and regulation of gene transcription.
Cramer P. (2019)
22 more

1 mention without citation

Structural advances in transcription elongation.
Mohamed et al. (2022)