› 5okf

X-ray diffraction
3.2Å resolution

CH1 chimera of human 14-3-3 sigma with the HSPB6 phosphopeptide in a conformation with self-bound phosphopeptides

Released:
DOI: 10.2210/pdb5okf/pdb
PDB entry 5okf coloured by chain, front view.
PDB entry 5okf coloured by chain, side view.
PDB entry 5okf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners.
Sluchanko NN, Tugaeva KV, Greive SJ, Antson AA
Sci Rep 7 12014 (2017)
PMID: 28931924

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127003 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
14-3-3 protein sigma; Heat shock protein beta-6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 246 amino acids
Theoretical weight: 27.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
  • Canonical: O14558 (Residues: 12-19; Coverage: 5%)
Gene names: HME1, HSPB6, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain

Ligands and Environments

1 bound ligand:
Ligand CD 17 x CD
2 modified residues:
Ligand CSO 4 x CSO
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 77.399Å b: 97.76Å c: 158.831Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.247 0.279
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

The Mechanism of SARS-CoV-2 Nucleocapsid Protein Recognition by the Human 14-3-3 Proteins.
Tugaeva et al. (2021)
7 more

2 mentions without citation

Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners.
Sluchanko et al. (2017)
1 more