› 5oma

X-ray diffraction
3.9Å resolution

CH3 chimera of human 14-3-3 sigma with the StARD1 peptide including Ser57

Released:
DOI: 10.2210/pdb5oma/pdb
PDB entry 5oma coloured by chain, front view.
PDB entry 5oma coloured by chain, side view.
PDB entry 5oma coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners.
Sluchanko NN, Tugaeva KV, Greive SJ, Antson AA
Sci Rep 7 12014 (2017)
PMID: 28931924

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152121 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma; Steroidogenic acute regulatory protein, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 27.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
  • Canonical: P49675 (Residues: 54-62; Coverage: 3%)
Gene names: HME1, SFN, STAR, STARD1
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Undetermined peptide Chain: H
Molecule details ›
Chain: H
Length: 4 amino acids
Theoretical weight: 358 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand TRS 1 x TRS
Ligand PO4 1 x PO4
Ligand SO4 1 x SO4
1 modified residue:
Ligand SEP 8 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 123.251Å b: 123.251Å c: 162.447Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.248
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

The Mechanism of SARS-CoV-2 Nucleocapsid Protein Recognition by the Human 14-3-3 Proteins.
Tugaeva et al. (2021)
8 more

1 mention without citation

Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners.
Sluchanko et al. (2017)