5ona

X-ray diffraction
2.7Å resolution

Drosophila Bag-of-marbles CBM peptide bound to human CAF40-CNOT1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-107786 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CCR4-NOT transcription complex subunit 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 244 amino acids
Theoretical weight: 27.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5YKK6 (Residues: 1351-1588; Coverage: 10%)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Sequence domains: CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
CCR4-NOT transcription complex subunit 9 Chains: B, E
Molecule details ›
Chains: B, E
Length: 273 amino acids
Theoretical weight: 31.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92600 (Residues: 19-285; Coverage: 89%)
Gene names: CNOT9, RCD1, RQCD1
Sequence domains: Cell differentiation family, Rcd1-like
Structure domains: Leucine-rich Repeat Variant
Protein bag-of-marbles Chains: C, F
Molecule details ›
Chains: C, F
Length: 24 amino acids
Theoretical weight: 2.87 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: P22745 (Residues: 13-36; Coverage: 5%)
Gene names: CG10422, bam

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 106.6Å b: 106.6Å c: 263.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided