› 5onf

X-ray diffraction
2.8Å resolution

The ENTH domain from epsin-1

Released:
DOI: 10.2210/pdb5onf/pdb
PDB entry 5onf coloured by chain, front view.
PDB entry 5onf coloured by chain, side view.
PDB entry 5onf coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Epsin and Sla2 form assemblies through phospholipid interfaces.
Garcia-Alai MM, Heidemann J, Skruzny M, Gieras A, Mertens HDT, Svergun DI, Kaksonen M, Uetrecht C, Meijers R
Nat Commun 9 328 (2018)
PMID: 29362354

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-171371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epsin-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 154 amino acids
Theoretical weight: 18.01 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12518 (Residues: 1-154; Coverage: 34%)
Gene names: ENT1, YDL161W
Sequence domains: ENTH domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

1 bound ligand:
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2221
Unit cell:
a: 68.929Å b: 119.363Å c: 129.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.272 0.285
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Novel roles of phosphoinositides in signaling, lipid transport, and disease.
Hammond et al. (2020)
2 more

3 mentions without citation

Epsin and Sla2 form assemblies through phospholipid interfaces.
Garcia-Alai et al. (2018)
2 more