› 5owo

X-ray diffraction
1.79Å resolution

Human cytoplasmic Dynein N-Terminus dimerization domain at 1.8 Angstrom resolution

Released:
DOI: 10.2210/pdb5owo/pdb
PDB entry 5owo coloured by chain, front view.
PDB entry 5owo coloured by chain, side view.
PDB entry 5owo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM shows how dynactin recruits two dyneins for faster movement.
Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP
Nature 554 202-206 (2018)
PMID: 29420470

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytoplasmic dynein 1 heavy chain 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 201 amino acids
Theoretical weight: 22.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14204 (Residues: 1-201; Coverage: 4%)
Gene names: DHC1, DNCH1, DNCL, DNECL, DYHC, DYNC1H1, KIAA0325

Ligands and Environments

5 bound ligands:
Ligand GOL 9 x GOL
Ligand MG 26 x MG
Ligand CA 2 x CA
Ligand NA 1 x NA
Ligand K 1 x K
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 101.242Å b: 175.56Å c: 50.444Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.229
Expression system: Escherichia coli

Quick links

Citations

21 review citations

The cytoplasmic dynein transport machinery and its many cargoes.
Reck-Peterson et al. (2018)
20 more

2 mentions without citation

Structure of the dynein-2 complex and its assembly with intraflagellar transport trains.
Toropova et al. (2019)
1 more