5pb3

X-ray diffraction
1.9Å resolution

Crystal Structure of Factor VIIa in complex with 1-[[3-[4-(5-amino-1H-pyrrolo[3,2-b]pyridin-2-yl)-5-hydroxypyrazol-1-yl]phenyl]methyl]-3-phenylurea

Released:
Source organism: Homo sapiens
Entry authors: Stihle M, Mayweg A, Roever S, Rudolph MG

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-140351 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Factor VII light chain Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 6.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08709 (Residues: 149-212; Coverage: 14%)
Gene name: F7
Sequence domains: Coagulation Factor Xa inhibitory site
Structure domains: Laminin
Factor VII heavy chain Chain: C
Molecule details ›
Chain: C
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41212
Unit cell:
a: 95.192Å b: 95.192Å c: 116.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.211
Expression system: Escherichia coli