› 5svl

X-ray diffraction
2.9Å resolution

Crystal structure of the ATP-gated human P2X3 ion channel in the ATP-bound, closed (desensitized) state

Released:
DOI: 10.2210/pdb5svl/pdb
PDB entry 5svl coloured by chain, front view.
PDB entry 5svl coloured by chain, side view.
PDB entry 5svl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structures define human P2X(3) receptor gating cycle and antagonist action.
Mansoor SE, Lü W, Oosterheert W, Shekhar M, Tajkhorshid E, Gouaux E
Nature 538 66-71 (2016)
PMID: 27626375

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
P2X purinoceptor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 40.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P56373 (Residues: 6-364; Coverage: 90%)
Gene name: P2RX3
Sequence domains: ATP P2X receptor
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
6 bound ligands:
Ligand ATP 2 x ATP
Ligand NAG 6 x NAG
Ligand EDO 6 x EDO
Ligand ACT 3 x ACT
Ligand NA 3 x NA
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P213
Unit cell:
a: 172.14Å b: 172.14Å c: 172.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.24
Expression system: Homo sapiens

Quick links

Citations

36 review citations

The P2X7 Receptor in Infection and Inflammation.
Di Virgilio et al. (2017)
35 more

12 mentions without citation

The Use of Defined Microbial Communities To Model Host-Microbe Interactions in the Human Gut.
Elzinga et al. (2019)
11 more