› 5svm

X-ray diffraction
3.09Å resolution

Crystal structure of the ATP-gated human P2X3 ion channel bound to agonist 2-methylthio-ATP in the desensitized state

Released:
DOI: 10.2210/pdb5svm/pdb
PDB entry 5svm coloured by chain, front view.
PDB entry 5svm coloured by chain, side view.
PDB entry 5svm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structures define human P2X(3) receptor gating cycle and antagonist action.
Mansoor SE, Lü W, Oosterheert W, Shekhar M, Tajkhorshid E, Gouaux E
Nature 538 66-71 (2016)
PMID: 27626375

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
P2X purinoceptor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 40.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P56373 (Residues: 6-364; Coverage: 90%)
Gene name: P2RX3
Sequence domains: ATP P2X receptor
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand NAG 6 x NAG
Ligand 6AT 1 x 6AT
Ligand NA 2 x NA
Ligand TRS 1 x TRS
Ligand EDO 2 x EDO
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P213
Unit cell:
a: 172.64Å b: 172.64Å c: 172.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.228
Expression system: Homo sapiens

Quick links

Citations

38 review citations

The P2X7 Receptor in Infection and Inflammation.
Di Virgilio et al. (2017)
37 more

3 mentions without citation

X-ray structures define human P2X(3) receptor gating cycle and antagonist action.
Mansoor et al. (2016)
2 more