› 5svo

X-ray diffraction
1.87Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP], mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 424 amino acids
Theoretical weight: 48.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48735 (Residues: 40-452; Coverage: 91%)
Gene name: IDH2
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 58.661Å b: 119.207Å c: 127.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.218
Expression system: Escherichia coli

Quick links

Citations

8 review citations

2-Hydroxyglutarate in Cancer Cells.
Ježek P. (2020)
7 more

2 mentions without citation

Point Mutation Specific Antibodies in B-Cell and T-Cell Lymphomas and Leukemias: Targeting IDH2, KRAS, BRAF and Other Biomarkers RHOA, IRF8, MYD88, ID3, NRAS, SF3B1 and EZH2.
Singh et al. (2021)
1 more