› 5svq

X-ray diffraction
3.25Å resolution

Crystal structure of the ATP-gated human P2X3 ion channel bound to competitive antagonist TNP-ATP

Released:
DOI: 10.2210/pdb5svq/pdb
PDB entry 5svq coloured by chain, front view.
PDB entry 5svq coloured by chain, side view.
PDB entry 5svq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structures define human P2X(3) receptor gating cycle and antagonist action.
Mansoor SE, Lü W, Oosterheert W, Shekhar M, Tajkhorshid E, Gouaux E
Nature 538 66-71 (2016)
PMID: 27626375

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
P2X purinoceptor 3 Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 40.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P56373 (Residues: 6-364; Coverage: 90%)
Gene name: P2RX3
Sequence domains: ATP P2X receptor
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 3 x NAG
Ligand NA 1 x NA
Ligand MG 1 x MG
Ligand 128 1 x 128
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 120.45Å b: 120.45Å c: 235.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.249 0.289
Expression system: Homo sapiens

Quick links

Citations

37 review citations

The P2X7 Receptor in Infection and Inflammation.
Di Virgilio et al. (2017)
36 more

8 mentions without citation

Molecular Pharmacology of P2X Receptors: Exploring Druggable Domains Revealed by Structural Biology.
Oken et al. (2022)
7 more