› 5svz

X-ray diffraction
2Å resolution

HIV-1 Tat NLS in complex with importin alpha

Released:
DOI: 10.2210/pdb5svz/pdb
PDB entry 5svz coloured by chain, front view.
PDB entry 5svz coloured by chain, side view.
PDB entry 5svz coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Importin-α Binding of the Human Immunodeficiency Virus Tat.
Smith KM, Himiari Z, Tsimbalyuk S, Forwood JK
Sci Rep 7 1650 (2017)
PMID: 28490747

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137594 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 55.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Protein Tat Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.9 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04326 (Residues: 48-60; Coverage: 15%)
Gene name: tat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 79Å b: 89.831Å c: 99.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Genetic variation and function of the HIV-1 Tat protein.
Spector et al. (2019)
4 more

3 mentions without citation

Structural Basis for Importin-α Binding of the Human Immunodeficiency Virus Tat.
Smith et al. (2017)
2 more