› 5t0n

X-ray diffraction
3Å resolution

Pseudo-apo structure of Sestrin2 at 3.0 angstrom resolution

Released:
DOI: 10.2210/pdb5t0n/pdb
PDB entry 5t0n coloured by chain, front view.
PDB entry 5t0n coloured by chain, side view.
PDB entry 5t0n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The apo-structure of the leucine sensor Sestrin2 is still elusive.
Saxton RA, Knockenhauer KE, Schwartz TU, Sabatini DM
Sci Signal 9 ra92 (2016)
PMID: 27649739

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157638 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sestrin-2 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 480 amino acids
Theoretical weight: 54.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P58004 (Residues: 1-480; Coverage: 100%)
Gene names: Hi95, SESN2, SEST2
Sequence domains: PA26 p53-induced protein (sestrin)

Ligands and Environments

1 bound ligand:
Ligand LEU 5 x LEU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I23
Unit cell:
a: 292.263Å b: 292.263Å c: 292.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Nutrient sensing and TOR signaling in yeast and mammals.
González et al. (2017)
11 more

1 mention without citation

The apo-structure of the leucine sensor Sestrin2 is still elusive.
Saxton et al. (2016)