5t6z

X-ray diffraction
2Å resolution

KIR3DL1 in complex with HLA-B*57:01-TW10

Released:

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-135135 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Capsid protein p24 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.16 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P03366 (Residues: 240-249; Coverage: 1%)
Gene name: gag-pol
Killer cell immunoglobulin-like receptor 3DL1 Chain: G
Molecule details ›
Chain: G
Length: 299 amino acids
Theoretical weight: 33.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P43629 (Residues: 22-320; Coverage: 71%)
Gene names: CD158E, KIR3DL1, NKAT3, NKB1
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 51.812Å b: 61.382Å c: 65.359Å
α: 95.31° β: 98.02° γ: 109.23°
R-values:
R R work R free
0.205 0.203 0.24
Expression systems:
  • Escherichia coli BL21
  • Not provided
  • Homo sapiens