› 5t7h

X-ray diffraction
2Å resolution

Crystal structure of dimeric yeast iso-1-cytochrome C with CYMAL6

Released:
DOI: 10.2210/pdb5t7h/pdb
PDB entry 5t7h coloured by chain, front view.
PDB entry 5t7h coloured by chain, side view.
PDB entry 5t7h coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cytochrome c Can Form a Well-Defined Binding Pocket for Hydrocarbons.
McClelland LJ, Steele HB, Whitby FG, Mou TC, Holley D, Ross JB, Sprang SR, Bowler BE
J Am Chem Soc 138 16770-16778 (2016)
PMID: 27990813

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c isoform 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 109 amino acids
Theoretical weight: 12.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00044 (Residues: 1-109; Coverage: 100%)
Gene names: CYC1, J1653, YJR048W
Sequence domains: Cytochrome c

Ligands and Environments

3 bound ligands:
Ligand SO4 9 x SO4
Ligand ZE7 4 x ZE7
Ligand HEC 4 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P1
Unit cell:
a: 50.351Å b: 56.122Å c: 56.127Å
α: 75.65° β: 63.37° γ: 63.36°
R-values:
R R work R free
0.202 0.198 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

MOMP, cell suicide as a BCL-2 family business.
Kalkavan et al. (2018)
1 more

1 mention without citation

Cytochrome c Can Form a Well-Defined Binding Pocket for Hydrocarbons.
McClelland et al. (2016)