› 5t8r

X-ray diffraction
2.4Å resolution

Crystal structure of human BAZ2A PHD zinc finger in complex with unmodified H3 10-mer

Released:
DOI: 10.2210/pdb5t8r/pdb
PDB entry 5t8r coloured by chain, front view.
PDB entry 5t8r coloured by chain, side view.
PDB entry 5t8r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of molecular recognition of helical histone H3 tail by PHD finger domains.
Bortoluzzi A, Amato A, Lucas X, Blank M, Ciulli A
Biochem J 474 1633-1651 (2017)
PMID: 28341809

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
monomeric
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159448 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain adjacent to zinc finger domain protein 2A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 58 amino acids
Theoretical weight: 6.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UIF9 (Residues: 1673-1728; Coverage: 3%)
Gene names: BAZ2A, KIAA0314, TIP5
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1 Chains: E, G
Molecule details ›
Chains: E, G
Length: 10 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-11; Coverage: 7%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand PO4 2 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 72.607Å b: 72.607Å c: 99.431Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

DNA Methylation Readers and Cancer: Mechanistic and Therapeutic Applications.
Mahmood et al. (2019)
4 more

4 mentions without citation

Structural basis of molecular recognition of helical histone H3 tail by PHD finger domains.
Bortoluzzi et al. (2017)
3 more