› 5tbm

X-ray diffraction
1.85Å resolution

Crystal structure of PT2385 bound to HIF2a-B*:ARNT-B* complex

Released:
DOI: 10.2210/pdb5tbm/pdb
PDB entry 5tbm coloured by chain, front view.
PDB entry 5tbm coloured by chain, side view.
PDB entry 5tbm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Small-Molecule Antagonist of HIF2α Is Efficacious in Preclinical Models of Renal Cell Carcinoma.
Wallace EM, Rizzi JP, Han G, Wehn PM, Cao Z, Du X, Cheng T, Czerwinski RM, Dixon DD, Goggin BS, Grina JA, Halfmann MM, Maddie MA, Olive SR, Schlachter ST, Tan H, Wang B, Wang K, Xie S, Xu R, Yang H, Josey JA
Cancer Res 76 5491-500 (2016)
PMID: 27635045

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151026 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endothelial PAS domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99814 (Residues: 237-348; Coverage: 13%)
Gene names: BHLHE73, EPAS1, HIF2A, MOP2, PASD2
Sequence domains: PAS domain
Structure domains: PAS domain
Aryl hydrocarbon receptor nuclear translocator Chain: B
Molecule details ›
Chain: B
Length: 117 amino acids
Theoretical weight: 13.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27540 (Residues: 354-467; Coverage: 14%)
Gene names: ARNT, BHLHE2
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand 79A 1 x 79A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 73.4Å b: 84.097Å c: 41.401Å
α: 90° β: 106.24° γ: 90°
R-values:
R R work R free
0.241 0.238 0.3
Expression system: Escherichia coli

Quick links

Citations

71 review citations

Glutamine reliance in cell metabolism.
Yoo et al. (2020)
70 more

6 mentions without citation

Hypoxia inducible factor (HIF) as a model for studying inhibition of protein-protein interactions.
Burslem et al. (2017)
5 more