› 5tc5

X-ray diffraction
1.96Å resolution

Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with butylthio-DADMe-Immucillin-A and chloride

Released:
DOI: 10.2210/pdb5tc5/pdb
PDB entry 5tc5 coloured by chain, front view.
PDB entry 5tc5 coloured by chain, side view.
PDB entry 5tc5 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cameron SA, Firestone RS, Schramm VL, Almo SC

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 297 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13126 (Residues: 1-283; Coverage: 100%)
Gene names: MSAP, MTAP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand BIG 3 x BIG
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I222
Unit cell:
a: 89.522Å b: 121.927Å c: 192.522Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.217
Expression system: Escherichia coli BL21(DE3)

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