› 5tc7

X-ray diffraction
1.75Å resolution

Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with 5'-methylthiotubercidin at 1.75 angstrom

Released:
DOI: 10.2210/pdb5tc7/pdb
PDB entry 5tc7 coloured by chain, front view.
PDB entry 5tc7 coloured by chain, side view.
PDB entry 5tc7 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cameron SA, Firestone RS, Schramm VL, Almo SC

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13126 (Residues: 1-283; Coverage: 100%)
Gene names: MSAP, MTAP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand PO4 3 x PO4
Ligand MTH 1 x MTH
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P321
Unit cell:
a: 123.359Å b: 123.359Å c: 44.499Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.17 0.184
Expression system: Escherichia coli BL21(DE3)

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