› 5tc8

X-ray diffraction
1.8Å resolution

Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with methylthio-DADMe-Immucillin-A

Released:
DOI: 10.2210/pdb5tc8/pdb
PDB entry 5tc8 coloured by chain, front view.
PDB entry 5tc8 coloured by chain, side view.
PDB entry 5tc8 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cameron SA, Firestone RS, Schramm VL, Almo SC

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13126 (Residues: 1-283; Coverage: 100%)
Gene names: MSAP, MTAP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

6 bound ligands:
Ligand TDI 1 x TDI
Ligand PO4 1 x PO4
Ligand MG 2 x MG
Ligand CL 1 x CL
Ligand 2PE 1 x 2PE
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P321
Unit cell:
a: 123.065Å b: 123.065Å c: 44.755Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links