› 5tcx

X-ray diffraction
2.96Å resolution

Crystal structure of human tetraspanin CD81

Released:
DOI: 10.2210/pdb5tcx/pdb
PDB entry 5tcx coloured by chain, front view.
PDB entry 5tcx coloured by chain, side view.
PDB entry 5tcx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of a Full-Length Human Tetraspanin Reveals a Cholesterol-Binding Pocket.
Zimmerman B, Kelly B, McMillan BJ, Seegar TCM, Dror RO, Kruse AC, Blacklow SC
Cell 167 1041-1051.e11 (2016)
PMID: 27881302

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CD81 antigen Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P60033 (Residues: 2-236; Coverage: 100%)
Gene names: CD81, TAPA1, TSPAN28
Sequence domains: Tetraspanin family

Ligands and Environments

1 bound ligand:
Ligand CLR 1 x CLR
1 modified residue:
Ligand YCM 2 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 101.43Å b: 73.51Å c: 70.35Å
α: 90° β: 125.11° γ: 90°
R-values:
R R work R free
0.27 0.267 0.294
Expression system: Spodoptera frugiperda

Quick links

Citations

57 review citations

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van Niel et al. (2018)
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38 mentions without citation

Applications of sequence coevolution in membrane protein biochemistry.
Nicoludis et al. (2018)
37 more